Journal: Journal of Sport and Health Science

Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

doi: 10.1016/j.jshs.2025.101095

Figure Lengend Snippet: Impact of high fat feeding and voluntary exercise on left ventricle morphology in male mice. (A) Representative images of Picrosirius red staining for measurement of interstitial and perivascular fibrosis with 20× magnification. (B) Representative images for Hematoxylin and Eosin for measurement of cardiomyocyte width and area with 40× magnification. (C) Representative images of Oil red O staining for measurement of interstitial lipid deposition with 40× magnification. Quantitative analysis of percent area of (D) interstitial and (E) perivascular fibrosis expressed as fold change from sedentary chow group. Quantitative analysis of (F) cardiomyocyte width and (G) cardiomyocyte area. (H) Quantitative analysis of percent area of lipid deposition expressed as fold change from sedentary chow group left ventricle mRNA expression of fatty acid transporters (I) FABP3, (J) CD36, and hypertrophy markers (K) β-MHC and (L) ANP in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (D–H) n : 8–12 per group. (I–L) n : 8–9 per group. * p < 0.05, **** p < 0.0001. ANP = atrial natriuretic peptide; β-MHC = beta myosin heavy chain; CD36 = platelet glycoprotein 4; FABP3 = fatty acid-binding protein 3; HFD = high fat diet; Myh7 = moysin heavy chain 7; VET = voluntary exercise training.

Article Snippet: The following TaqMan assay gene transcripts were used: fatty acid-binding protein 3 (FABP3, Mm02342495_m1), platelet glycoprotein 4 (CD36, Mm00432403_m1), beta myosin heavy chain (β-MHC, Mm00600555_m1), atrial natriuretic peptide (ANP, Mm01255747_g1), OPA1 (Mm01349707_g1), DRP1 (Mm01342903_m1), robosomal18S (18S, Mm03928990_g1).

Techniques: Staining, Expressing, Binding Assay

Journal: Molecular Biomedicine

Article Title: Curcumin alleviates metabolic dysfunction-associated steatotic liver disease via gut microbiota-dependent epigenetic alterations

doi: 10.1186/s43556-026-00606-1

Figure Lengend Snippet: C. sensu regulated lipid accumulation in hepatocytes in a CEBPB-dependent manner. a-d Representative BODIPY 493/503 fluorescence staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or control and transfected with siRNAs against CEBPB (si- CEBPB ) or negative control siRNA (si-NC) with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). e–f Quantification of intracellular TG content in HepG2 and Huh-7 cells treated with C. sensu or control transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM, n = 3). g-j Representative ORO staining of lipid droplet accumulation and quantitative analysis in HepG2 and Huh-7 cells treated with C. sensu or Control and transfected with si- CEBPB or si-NC, with or without OA (0.6 mM) treatment (scale bar, 100 μm, n = 3). k The levels of lipid transport- and synthesis-related proteins ( ACC1 , CD36 , and SCD1 ) in C. sensu - or control-treated HepG2 and Huh-7 cells transfected with si- CEBPB or si-NC with or without OA treatment (0.6 mM) were assessed by Western blotting ( n = 3). Results were presented as mean ± SD from three independent experiments. P values were calculated with one-way ANOVA followed by Tukey’s test. The abbreviation C. sensu represents Clostridium_sensu_stricto_1 . * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: The primary antibodies used were as follows: AHR (67785–1-Ig, Proteintech, China), CEBPB (23431–1-AP, Proteintech, China), ACC1 (21923–1-AP, Proteintech, China), CD36 (18836–1-AP, Proteintech, China), SCD1 (28678–1-AP, Proteintech, China), phosphorylated PI3K (p-PI3K, 138364, Abcam, USA), PI3K (4257, CST, USA), phosphorylated AKT (p-AKT, 66444–1-Ig, Proteintech, China), AKT (10176–2-AP, Proteintech, China), and β-actin (66009–1-Ig, Proteintech, China).

Techniques: Fluorescence, Staining, Analysis, Control, Transfection, Negative Control, Western Blot